The 4th Biomedical Imaging and Sensing Conference 2018(BISC2018)


April 25 Wed.

OMC&BISC1: OMC and BISC Joint Session 1
Chair: Takashige Omatsu(Chiba University, Japan)

Room 418

Start End Session No. Title Speaker Description
13:30 14:00 OMC&BISC1-1Plenary High Temporal and Spatial Pattern Stimulation to Manipulate  Brain Function Hiroaki Wake1, Koichiro Haruwaka1, Xiangyu Quan2, Osamu Matoba2
1Kobe University of Graduate School of Medicine, Japan,
2Kobe University, Japan
In the central nervous system (CNS), glial cells, originally termed “nervenkitt,” recently focused because of the understanding of their physiological functions. Here, we focused how glial cell regulates the function of neuronal circuits using in vivo two photon microscope.
14:00 14:30 OMC&BISC1-2Plenary Femtosecond Laser Trapping, Assembling, and Ejection Dynamics of Dielectric Nanoparticles in Solution Hiroshi Masuhara1, J. Chen1, W. Chiang1, 2, A. Usman3, T. Sugiyama1, 4, J. Hofkens2
1National Chiao Tung University, Taiwan,
2Katholieke Universiteit Leuven, Belgium,
3Universiti Brunei Darussalam, Brunei Darussalam,
4Nara Institute of Science and Technology, Japan
We study femtosecond laser trapping dynamics of Rayleigh particles by examining polystyrene, silica, and surfacemodified silica nanoparticles with different diameter and by changing solvent viscosity.
14:30 15:00 OMC&BISC1-3Plenary Monitoring Ruffling Cells by Lattice Light-sheet Microscopy Bi-Chang Chen, Wei-Chun Tang, Peilin Chen
Academia Sinica, Taiwan
The membrane ruffling can be triggered by rapamycin and/or blue light. After stimulation, the three- dimensional dynamics of membrane ruffling has been recorded by lattice light-sheet microscope (LLSM), which is capable of high spatial and temporal recording over three-dimensions.
15:00 15:30 Break

OMC&BISC2: OMC and BISC Joint Session 2
Chair: Osamu Matoba(Kobe University, Japan)

Room 418

Start End Session No. Title Speaker Description
15:30 15:45 OMC&BISC2-1Invited Computational Imaging and Reconstruction in Digital Holographic Microscopy Edmund Y.M. Lam
University of Hong Kong, Hong Kong
Digital holographic microscopy captures the 3D information of biological specimens as holograms, which can then be reconstructed into sectional images computationally. In this talk, we will discuss recent advances in such algorithms and applications.
15:45 16:00 OMC&BISC2-2 Observation of Immunostained Microtubules Using Three-Dimensional Superresolution Microscope with Two-Color Annular Wave Plate Yoshinori Iketaki1, B. Nandor2, D. Okada3, H. Kumagai4
1Olympus Corp, Japan,
2Budapest University of Technology and Economics, Hungary,
3Kitasato University, School of Medicine, Japan,
4Kitasato University, School of Allied Health Sciences Physics, Japan
Three-dimensional super-resolution microscopy based on fluorescence depletion (3D-SRM) was applied to the observation of immunostained microtubules having complicated structures stacking each other.
16:00 16:15 OMC&BISC2-3 Optical Trapping of Quantum-Dot Conjugated AMPA-type Receptors Depened on Initial Assembling States Tatsunori Kishimoto1, 2, Y. Maezawa1, S. N. Kudoh2, T. Taguchi3, C. Hosokawa1, 2, 4
1National Institute of Advanced Industrial Science and Technology (AIST), Japan,
2Kwansei Gakuin University, Japan,
3National Institute of Information and Communications Technology (NICT), Japan,
4Advanced Photonics and Biosensing Open Innovation Laboratory, AIST-Osaka University, Japan
AMPA-type glutamate receptor (AMPAR) is one of the major neurotransmitter receptors at excitatory synapses.
16:15 16:30 OMC&BISC2-4 Enhanced Collection Efficiency of Vesicles in A Suspension by Optical Pressure Using Gold Nanoparticles Takashi Kaneta, M. Kuboi, N. Takeyasu
Okayama University, Japan
We describe a collection of micro-vesicles on a glass substrate using the optical pressure of a laser beam. The laser beam was focused on the glass substrate which sandwiches a suspension containing micrometer-sized vesicles prepared by a phospholipid.
16:30 16:45 OMC&BISC2-5 Rotational Dynamics of Bacteria in An Optical Tweezer Sharath Ananthamurthy
University of Hyderabad, India
The swimming of bacterium in fluid occurs in low-Reynold’s regime. The trapping of a bacterium using laser light gives us information about the hydrodynamic coefficients which are very important in understanding the efficiency of swimming of bacterium.
April 26 Thu.

BISC1: Advanced Optical Miroscopy 1
Chair: Toyohiko Yatagai(Utsunomiya University, Japan)

Room 419

Start End Session No. Title Speaker Description
9:30 9:45 Opening
Toyohiko Yatagai
Utsunomiya University, Japan
9:45 10:15 BISC1-1
Coherent brightfield (COBRI) micros copy for ultrahig h-speed single- particle tracking on lipid bilayer membranes Yi-Hung Liao, Chia-Lung Hsie
Academia Sinica, Taiwan
Detecting linear scattering light from biological entity is useful for label-free bioimaging. Coherent brightfield (COBRI) microscopy provides the sensitivity to see individual native biological nanoparticles in live cells with nanometer spatial localization precision and microsecond temporal resolution.
10:15 10:45 BISC1-2
Visualizing a beating zebrafish heart with improved line-scan microscopy Nanguang Chen
National Univ of Singapore, Singapore
We have developed a high-speed line-scan optical microscope that is capable of acquiring high-resolution, high-contrast fluorescence images at more than 100 fps. This advanced imaging technique has been applied to multi-dimensional imaging of zebrafish heart. 
10:45 11:00 Break
11:00 11:30 BISC1-3
Fast, long-term super-resolution imaging with Hessian structured illumination microscopy Xiaoshuai Huang1, Junchao Fan2, Liuju Li1, Haosen Liu2, Runlong Wu3, Yi Wu4, Lisi Wei1, Heng Mao5, Amit Lal6, Peng Xi6, Liqiang Tang7, Yunfeng Zhang3, Yanmei Liu1, Shan Tan2, Liangyi Chen1
1Institute of Molecular Medicine, Peking University, China,
2Huazhong University of Science and Technology, China,
3School of Electronics Engineering and Computer Science, Peking University, China,
4School of Software and Microelectronics, Peking University, China,
5School of Mathematical Sciences, Peking University, China,
6Department of Biomedical Engineering, Peking University, China,
7ColdSpring Science Corporation, China
To increase the temporal resolution and maximal imaging time of super-resolution (SR) microscopy, we have developed a deconvolution algorithm for structured illumination microscopy based on Hessian matrixes (Hessian-SIM).
11:30 11:45 BISC1-4 Multi-focal holographic slit confocal fluorescence microscopy Hung-Chun Wang1, Yuan Luo2, Chou-Min Chia2
1National Tsing Hua University, Taiwan,
2National Taiwan University, Taiwan
The non-axial line-scanning multifocal confocal microscopy incorporates multiplex volume holographic grating (MVHG) in illumination and combination of multifocal image system to image multiplane at the same time. The system resolve volume tissue fast and well, offering a solution of replacing biopsy.
11:45 12:00 BISC1-5 Lateral spatial resolution improvement in laser scanning fluorescence microscopy using a subdiffraction limit optical spo Takahiro Nishimura1, Yusuke Ogura1, Yosuke Tamada2, Jun Tanida1
1Osaka Univ, Japan,
2NIBB, Japan
This paper confirmed that use of a computer generated hologram that makes the size of the generated spots smaller than the single diffraction limited can improve the lateral spatial resolution of the laser scanning fluorescence microscopy.
12:00 13:15 Lunch

BISC2: Optical Coherence Tomography
Chair: Yuan Luo(National Taiwan University, Taiwan)

Room 419

Start End Session No. Title Speaker Description
13:15 13:45 BISC2-1
Endoscopic optical coherence tomography and angiography for gastroenterology applications Hsiang-Chieh Lee1, 2, Kaicheng Liang2, Osman O. Ahsen2, Zhao Wang2, Marisa Figueiredo3, Benjamin Potsaid2, 4, Vijaysekhar Jayaraman5, Qin Huang3, Hiroshi Mashimo3, 6, James G. Fujimoto2
1National Taiwan Univ, Taiwan,
2Massachusetts Institute of Technology, USA,
3VA Boston Healthcare System, USA,
4Thorlabs Inc., USA,
5Praevium Research Inc., USA,
6Harvard Medical School, USA
Endoscopic optical coherence tomography (OCT) angiography enables volumetric coregistered architectural and microvasculature imaging of the human gastrointestinal tract in vivo. In this talk, we will discuss technical advances and clinical gastroenterology applications with the endoscopic OCT angiography technique.
13:45 14:00 BISC2-2 Evaluation of photodamage with polarization-sensitive optical coherence tomography Trung Nguyen Hoang, Cheng Yu Lee, Meng-Tsan Tsai
Chang Gung University, Taiwan
Polarization sensitive optical coherence tomography (PS-OCT) is an extension of conventional optical coherence tomography (OCT) which enable the function to investigate birefringence characteristic of materials of biological tissue.
14:00 14:15 BISC2-3 A 2.8-mm beam diameter system for retinal imaging with OCT and adaptive optics Maddipatla Reddikumar, Joel Cervantes, Yukitoshi Otani, Barry Cense
Utsunomiya Univ, Taiwan
A less-complex 2.8-mm beam diameter spectral domain optical coherence tomography system with an adaptive optics module presented. In this system a Shack-Hartmann wavefront sensor used for aberration sensing and the Deformable mirror used for aberration correction.
14:15 14:30 BISC2-4 Study on photothermolysis with multi-functional, high-resolution optical coherence tomography Tai-Ang Wang1, Ming-Che Chan1, Meng-Tsan Tsai2
1National Chiao Tung University, Taiwan,
2Chang Gung University, Taiwan
A high-resolution OCT is developed for observation of photothemolysis effect. Using a compact supercontinuum generation laser to achieve high resolution, our HROCT is centered at 750nm, with 300nm bandwidth, and axial resolution < 1 micron.
14:30 14:45 BISC2-5 Motion artifacts in endoscopic catheter-based images: simulation and motion correction method Elham Abouei1, Anthony M. D. Lee2, Geoffrey Hohert2, Michelle C. Cua2, Pierre Lane2
1Univ of British Columbia, Canada,
2BC cancer research center, Canada
A model of motion artifacts for 3D/2D rotational pullback catheter data and a motion correction method called azimuthal en face image registration is presented. Qualitative and quantitative evaluations of the method are analysed for OCT-AFI images.
14:45 15:00 BISC2-6 Assessment of micro-optical coherence tomography (μOCT) as an imaging tool for pancreatic disease diagnosis Chi Hu1, Linbo Liu2
1Northwestern Polytechnical University, China,
2Nanyang Technological University, Singapore
The feasibility of µOCT as an imaging tool for pancreatic disease diagnosis is evaluated.Ex vivo experiments with both normal tissues and those with pancreatic edema are conducted to verify the applicability of µOCT
15:00 15:30 Break

BISC3: Optical Imaging of Multimodal and Biomedical Information
Chair: Tatsuki Tahara(Kansai Univ., Japan)

Room 419

Start End Session No. Title Speaker Description
15:30 16:00 BISC3-1
Spatial resolution enhancement in laser scanning microscopy using vector beams Yuichi Kozawa1, 2, Shunichi Sato1
1Tohoku Univ, Japan,
Cylindrical vector beams have unique features such as the formation of a small focal spot under tight focusing condition. We utilize such characteristic to enhance the spatial resolution in laser scanning microscopy.
16:00 16:30 BISC3-2
Computational imaging utilizing volume hologram Tomoya Nakamura, Masahiro Yamaguchi
Tokyo Institute of Technology, Japan
Volume hologram is a powerful tool for enabling computational imaging. This report mainly introduces the light-field imaging system utilizing volume hologram, and the talk corresponding to this report also addresses other applications of volume hologram
16:30 17:00 BISC3-3
Multimodal digital holographic microscopy for simultaneous phase and fluorescence imaging Xiangyu Quan1, Osamu Matoba1, Yasuhiro Awatsuji2
1Kobe Univ., Japan,
2Kyoto Inst. of Tech., Japan
This paper introduces a new type of multimodal digital holographic microscopy for biological applications. Off-axis digital holography is applied both in 3D phase imaging and 3D fluorescence imaging. In addition, image recovery by iterative method to obtain focused fluorescence images are introduced.
17:00 17:30 BISC3-4
Fluorescent molecular force probes for rheology and mechanobiology Shohei Saito
Kyoto Univ., Japan
Fluorescent molecular force probes have been developed for rheology and mechanobiology. To realize fluorescence response to pico-Newton forces, we have explored flexible force probes based on a conformational change of flexible molecules.
17:30 18:00 BISC3-5
Illuminating gene expression dynamics by optogenetics Akihiro Isomura
Kyoto Univ., Japan
Cells receive diverse signaling cues from their environment that trigger cascades of biochemical reactions in a dynamic manner. Live-cell imaging technologies have revealed dynamic patterns of gene activities; however it has been challenging to clarify….
April 27 Fri.

BISC4: Multimodal Imaging and Devices
Chair: Yoshihisa Aizu(Muroran Institute of Technology, Japan)

Room 419

Start End Session No. Title Speaker Description
9:00 9:15 BISC4-1 Non-scanning in-vivo three-dimensional hybrid structured illumination microscopy h-speed single- particle tracking on lipid bilayer membranes Ju-Hsuan Chien
National Taiwan University, Taiwan
Demonstrate HiLo structured illumination microscopy by the use of digital micro-mirror device (DMD) and the focal tunable lens (FTL). This system are high-resolution, wide-field optically sectioning and viewing in vivo biological tissue samples without mechanical scanning.
9:15 9:30 BISC4-2 Shape based pharmacokinetic fluorescence optical tomography Omprakash Gottam, Naren Naik
Indian Institute of Technology Kanpur, India
Spatially varying pharmacokinetic rates in a tissue help in identifying abnormal regions since they govern the exchange of fluorophore dye between blood plasma and tissue.
9:30 9:45 BISC4-3 Fast spatial domain reconstruction for structured illumination microscopy Xing Zhou, Dan Dan, Baoli Yao
Xi’an Institute of Optics and Precision Mechanics, China
As a wide-field super-resolution (SR) technique, structured illumination microscopy (SIM) features the merits of fast imaging speed, low excitation intensity and a large field of view. 
9:45 10:00 BISC4-4 Visible resonance Raman spectroscopy detect key molecular biomarker vibrations to characterize for human brain gliomas Cheng-Hui Liu1, Yan Zhou2, Binlin Wu3, Xinguang Yu4, Gangge Cheng2, Chunyuan Zhang1, Cuicui Lu5, Ke Zhu6, Robert R. Alfano1
1Institute for Ultrafast Spectroscopy and Lasers, USA,
2Air Force General Hospital, China,
3Southern Connecticut State University, USA,
4PLA General Hospital, China,
5Qian xuesen Laboratory of Space Technology, China,
6Institute of Physics, Chinese Academy of Sciences (CAS), China
Key Raman vibrational modes at 1129cm-1 and 1338cm-1 were observed to characterize gliomas using WITec300R visible resonance Raman (VRR) spectrometer. This work may aid neurosurgeons better decide surgical margins of cancers.
10:00 10:15 BISC4-5 Long-life plastic optical fiber probes for scanning near-field optical microscope Anton Smirnov, Giovanni Dietler, Sergey Sekatskii
Ecole Polytechnique Fédérale de Lausanne, Switzerland
Sharpened glass fiber SNOM probes have certain severe drawbacks. We are presenting alternative SNOM probes made from plastic optical fibers. These probes demonstrate an excellent performance in both topographical and optical channels after intense use.
10:15 10:30 BISC4-6 Effect of speckle pattern illumination on holographic recording and reconstructio Vinu R. V., Darshika Singh
Rakesh Kumar Singh, IIST, India
A speckle field illumination technique for recording and reconstruction of the complete wavefront is proposed and experimentally demonstrated and compare with conventional holographic approach. This technique is expected to play an important role in studying the polarization sensitive materials.
10:30 10:45 BISC4-7 Isotropic quantitative differential phase contrast microscopic imaging Yu-Zi Lin, Kuang-Yuh Huang
National Taiwan University, Taiwan
We propose a new illumination method to achieve isotropic differential phase contrast microscopic imaging efficiently. Recover quantitative phase image of thin transparent sample under 2-axis intensity measurements. Improve the accuracy and stability of phase recovery in conventional differential phase contrast microscopy.
10:45 11:15 Break

BISC5: Digital Holographic Microscopy
Chair: Wataru Watanabe(Ritsumeikan University)

Room 419

Start End Session No. Title Speaker Description
11:15 11:45 BISC5-1
Incoherent digital holography for biomedical imagin Joseph Rosen
Ben-Gurion Univ of the Negev, Israel
The evolution of the FINCH incoherent digital holography method is described. Following the review of FINCH, other recently developed self-reference single-channel incoherent hologram recorders, branched out from FINCH, are discussed and several biomedical-related applications are described.
11:45 12:00 BISC5-2 Investigations of wavelength resolution and adoptable phase shifts in phase-shifting color digital holography with 2pi ambiguity and wavelength-multiplexed images Tatsuki Tahara1, 2, Reo Otani3, Yasuhiko Arai1, Yasuhiro Takaki4
1Kansai Univ, Japan,
2PRESTO, Japan Science and Technology Agency, Japan,
4Kansai Univ, Japan
We investigate wavelength resolution and adoptable phase shifts in phase-shifting color digital holography with 2pi ambiguity and wavelength-multiplexed images. 
12:00 12:15 BISC5-3 Single-shot incoherent digital holography using parallel phase-shifting radial shearing interferometry Syogo Mochida1, Takahito Fukuda1, Yasuhiro Awatsuji1, Kenzo Nishio1, Osamu Matoba2
1Kyoto Institute of Technology, Japan,
2Kobe University, Japan
We propose single-shot incoherent digital holography using parallel phase-shifting radial shearing interferometry. We applied this technique to the three-dimensional measurement of objects illuminated by incoherent light and put on different depth positions.
12:15 13:00 Lunch

BISCp6: Poster Session

Exhibition Hall A

Start End Session No. Title Speaker Description
13:00 14:30 BISCp6-1 Non-invasive glucose monitoring based on optical coherent tomography Quoc-Hung Phan1Tseng-Lin Chen1, Yu-Lung Lo1, 2
1Department of Mechanical Engineering, National Cheng Kung University, Taiwan,
2Advanced Optoelectronic Technology Center, National Cheng Kung University, Taiwan
A novel technique based on optical coherence tomography (OCT) for noninvasive glucose monitoring is proposed. The feasibility of the proposed technique is demonstrated by detecting the glucose concentration of aqueous solution ranging from 0~4000 mg/dL with 0.02% lipofundin. 
BISCp6-2 Monitoring protein-related degeneration of Drosophila eyes with optical coherence tomography Chia-Heng Wu1, Meng-Tsan Tsai2
1Chang Gung Univ, Taiwan,
2Department of Electrical Engineering, Chang Gung University, Taiwan
In this study, we propose to use optical coherence tomography (OCT) for the study of protein-related degeneration of Drosophila eyes. With OCT, the bristles of Drosophila eye can be identified and different mutant Drosophila were scanned with OCT for investigation of progress of protein-related degeneration.
BISCp6-3 Parallel phase-shifting radial shearing interferometry and its numerical verification Syogo Mochida1, Takahito Fukuda1, Yasuhiro Awatsuji2, Kenzo Nishio3, Osamu Matoba4
1Department of Electronics, Graduate School of Science and Technology, Kyoto Institute of Technology, Japan,
2Faculty of Electrical and Electronics, Kyoto Institute of Technology, Japan,
3Advanced Technology Center, Kyoto Institute of Technology, Japan,
4Department of Systems Science, Graduate School of System Informatics, Kobe University, Japan
We propose parallel phase-shifting radial shearing interferometry for single-shot measurement of wavefront from an object. We numerically simulated and demonstrated the wavefront measurement of two particles assumed as object wave by using the proposed technique.
BISCp6-4 Motion-picture phase imaging by an integrated optical system of a parallel phase-shifting digital holographic microscope Kazuki Shimizu1, Takahito Fukuda1, Peng Xia2, Yasuhiro Awatsuji3, Kenzo Nishio4, Osamu Matoba5
1Department of Electronics, Graduate School of Science and Technology, Kyoto Institute of Technology, Japan,
2National Institute of Advanced Industrial Science and Technology, Japan,
3Kyoto Institute of Technology, Faculty of Electrical Engineering and Electronics, Japan,
4Advanced Technology Center, Kyoto Institute of Technology, Japan,
5Department of Systems Science, Graduate School of System Informatics, Kobe University, Japan
The authors have designed and constructed an integrated optical system of parallel phase-shifting digital holographic microscope. Motion-picture phase imaging of a dynamic minute transparent specimen at 1,000 fps has been demonstrated by the microscope.
BISCp6-5 Digital holographic microscopy using speckle illuminations and two-wavelength method Hideki Funamizu1, Jun Uozumi2, Yoshihisa Aizu3
1Yusei Onodera, Muroran Institute of Technology, Japan,
2Faculty of Engineering, Hokkai-Gakuen University, Japan,
3Muroran Institute of Technology, Japan
It has been known that spatial resolution of digital holographic microscopy (DHM) can be enhanced using speckle illuminations. In this study, we report the application of the two-wavelength method to DHM using speckle illuminations.
BISCp6-6 Tomographic phase imaging of RBCs in blood coagulation structures using digital holographic microscopy Hideki Funamizu, Ryoji Goto, Yoshihisa Aizu
Muroran Institute of Technology, Japan
Blood coagulation is an important role in hemostasis. In this study, to observe the degree of blood coagulation, we demonstrate a tomographic phase imaging of aggregation structures of RBCs using digital holographic microscopy.
BISCp6-7 Reconstruction of complex amplitude by lensless phase-shift digital holography through an opaque glass plate Akinori Igarashi, Wataru Watanabe
Ritsumeikan University, Japan
Optical imaging through diffusive or scattering media has attracted much attention. Digital holographic microscopy provides quantitative phase imaging thorough diffusive media. We experimentally reconstruct intensity and phase images of an object through an opaque ground glass screen by means of digital holography.
BISCp6-8 Digital holographic size measurement of Daphnia pulex Kota Sunayama1, Hitoshi Miyakawa2, Yoshio Hayasak2
1Center for Optical Research and Education (CORE), Utsunomiya University, Japan,
2Center for Bioscience Research and Education (CBRE), Utsunomiya University, Japan
Toxicity of chemical substances should be determined for protecting biological environment. A Daphnia pulex is one of the indicator organisms for searching the toxicity, because the shape is changed depending on the toxicity. Conventional method for its observation has been performed under suppression of its movement in a small thin room with an ordinary optical microscope.
BISCp6-9 Incoherent holographic imaging of subsurface structures with volume holographic gratings Yu-Hsin Chia1, Hung-Chun Wang2, Yuan Luo3
1Institute of Medical Device and Imaging, National Taiwan University, Taiwan,
2Department of Power Mechanical Engineering, National Tsing Hua University, Taiwan,
3Institute of Medical Device and Imaging, National Taiwan University, Taiwan
Under broadband illumination, a multiplane microscopy incorporating volume holographic gratings (VHGs) to observe three-dimensional structures of biological samples is presented. Here, we experimentally demonstrate this microscopic imaging capability to obtain multiple depth-resolved images of fine structures from eight depths in one shot.
BISCp6-10 In vivo time-series monitoring of dermal collagen fiber during skin burn healing using second-harmonic-generation microscopy Eiji Hase1, 2, Ryosuke Tanaka3, Shu-ichiro Fukushima3, Takeshi Yasui1, 3
1Tokushima University, Japan,
2JASRI/SPring-8, Japan,
3Osaka University, Japan
We applied second-harmonic-generation microscopy for in vivo imaging of healing process in animal skin burn and visualized decomposition, production, and growth of renewal collagen fibers as a series of time-lapse images in the same subject.
BISCp6-11 In vivo visualization of dermal collagen fibers in human skin using a photonic-crystal-fiber-coupled, hand-held second-harmonic-generation microscope Yuki Ogura, Kosuke Atsuta, Eiji Hase, Takeo Minamikawa, Takeshi Yasui
Tokushima Univ., Japan
We constructed a hand-held second-harmonic-generation microscope for in vivo monitoring of collagen fibers in human skin by fiber delivery of ultrashort pulse light in a large-mode-area photonic-crystal-fiber and a compact microscopy setup.
BISCp6-12 Analysis of collagen fiber orientation in biological tissues using polarization-resolved second-harmonic-generation microscopy Takuya Sakaue1, Eiji Hase2, Takeo Minamikawa3
1Tokushima Univ, Japan,
2Tokushima Univ, Japan, SPring-8(JASRI), Japan,
3Takeshi Yasui, Tokushima Univ, Japan
We constructed continuously-polarization-resolved SHG microscopy based on rapid polarization rotation every 15 degrees with electric-optic Pockells cell, and applied it for the quantitative analysis of collagen fiber orientation in biological tissues.
BISCp6-13 In situ monitoring of incised wound healing in animal model using second-harmonic-generation and third-harmonic-generation microscopy Shu-ichiro Fukushima1, Yuji Tanaka1, Eiji Hase2, 3, Kazuma Takeichi2, Takeshi Yasui1, 2
1Osaka University, Japan,
2Tokushima University, Japan,
3JASRI/SPring-8, Japan
We apply the combination of second-harmonic-generation microscopy with third-harmonic-generation microscopy for incised wound animal model, and visualize collagen dynamics during the wound healing process in time series in situ.
BISCp6-14 Resonance Raman and fluorescence spectroscopy to evaluate increased brain kynurenine pathway activity in samples from patients with Alzheimer’s Laura A. Sordillo, Lin Zhang, Lingyan Shi, Peter Sordillo, Robert Alfano
City College of New York, United States
Resonance Raman and fluorescence spectroscopy were used to assess increased kynurenine pathway activity in brain samples from Alzheimer’s patients and age-matched controls. Increased activity was seen in areas of the brain involved in Alzheimer’s disease.
BISCp6-15 Development of dual-wavelength microscopic laser speckle contrast imaging system Cheng-Yu Lee1, Meng-Tsan Tsai1, 2
1Chang Gung University, Taiwan,
2Chang Gung Memorial Hospital, Taiwan
Laser Speckle Contrast Imaging (LSCI), which used coherent light, has fully been used for observing blood flow due to its non-invasive, non-contact acquisition method. Generally, LSCI system uses just a single wavelength for measurement. In this research, first, considering the biological characteristics of different reflection rates and absorption, we use two lasers at 633nm and 855 nm and two CCD cameras to build a microscopic LSCI system.
BISCp6-16 Polarization-resolved second-harmonic-generation imaging of dermal collagen fiber in pre-wrinkled skin of ultraviolet-B-exposed mouse Shu-ichiro Fukushima1, Makoto Yonetsu1, Eiji Hase2, 3, Takeshi Yasui1, 2
1Osaka University, Japan,
2Tokushima University, Japan,
3JASRI/SPring-8, Japan
We investigate orientation change of dermal collagen fiber in pre-wrinkled skin of ultraviolet-B-exposed mouse skin using polarization-resolved second-harmonic-generation microscopy, and confirm that change of collagen fiber orientation is a trigger of wrinkling in photo-aged skin.
BISCp6-17 Effects of nitric oxide on cortical hemodynamic responses in the rat brain exposed to a shock wave Masaki Inaba1, Shunichi Sato2, Izumi Nishidate3
1Tokyo University of Agriculture and Technology, Japan,
2National Defense Medical College Research Institute, Japan,
3Tokyo University of Agriculture and Technology, Japan
We examied how the nitric oxide synthesis inhibition altered hemodynamic responses to a laser-induced shock wave(LISW) by diffuse reflectance spectroscopy. The results suggested that hemodynamic abnormalities, or transient hyperemia/hyperoxemia followed by persistent oligemia/hypoxemia, caused by an LISW in the rat cortex was associated with an increased nitric oxide production and its vasodilatory/vasoconstrictory effects.
BISCp6-18 Partial optical path length in the scalp in subject-specific head models for multi-distance probe configuration of near infrared spectroscopy Taku Yanagisawa1, Hiroshi Kawaguchi2, Eiji Okada3
1Department of Electronics and Electrical Engineering,Keio University, Japan,
2National Institute of Advanced Industrial Science and Technology, Japan,
3Department of Electronics and Electrical Engineering,Keio University, Japan
Light propagation in 45 subject specific head models was calculated to predict the spatial variability of the partial optical path length in the scalp and the weighting factor for the multi-distance probe configuration of NIRS.
BISCp6-19 Measurement of head phantom by functional near infrared imaging using multi-distance probe configuration Koki Hayabusa, Eiji Okada
Department of Electronics and Electrical Engineering, Keio University, Japan
The topographic image of the head phantom including the local absorption change in gray matter and global absorption change in the scalp was measured by functional near infrared imaging system using multi-distance probe configuration.
BISCp6-20 Diffuse light reflectometry for measuring scattering and absorption coefficients of a biological tissue Daiki Maeda, Toshiaki Iwai
Tokyo University of Agriculture and Technology, Japan
The optical properties, a scattering and an absorption coefficients, of biological tissues will be used to estimate quantitatively change in bioactivity. Diffuse light reflectometry has been investigated to measure the optical properties of biological tissues from the viewpoints of applicability and practicality.
BISCp6-21 Adjoint based Hessian evaluation for SPN modeled optical tomography Nishigandha Patil, Naren Naik
Indian Institute of Technology Kanpur, India
We present for the first time an adjoint based evaluation of the Hessian matrix for the SPN-approximation modeled forward operator in optical tomography. The Hessians so calculated are numerically validated with respect to finite difference calculations.
BISCp6-22 Three-dimensional beam focusing control for lensless vascular endoscopes using local wavefront control Masaki Hisaka
Osaka Electro-Communication Univ, Japan
The focusing beam generated by an optical fiber bundle having locally optical delay has been investigated to simplify the structure of vascular endoscopes. We have demonstrated the beam focusing and scanning using a liquid lens.
BISCp6-23 Application of scan-less two-dimensional confocal microscopy achieved by a combination of confocal slit with wavelength/space conversion Eiji Hase1, 2, Takeo Minamikawa1, 2, Yasuhiro Mizutani2, 3, Tetsuo Iwata2, 4, Hirotsugu Yamamoto2, 5, Takeshi Yasui2, 6
1Tokushima Univ., Japan,
2JST, ERATO MINOSHIMA Intelligent Optical Synthesizer (IOS), Japan,
3Osaka Univ., Japan,
4Tokushima Univ., Japan,
5Utsunomiya Univ., Japan,
6Tokushima Univ., Japan
Scan-less confocal laser microscope is achieved by a combination of confocal slit with wavelength/space conversion and is applied it to several imaging to demonstrate its high potential.
BISCp6-24 Multi-focal imaging system by using a programmable spatial light modulator Chen Yen Lin, Yuan Luo
National Taiwan Univ, Taiwan
We have demonstrated that the multiplexed gratings pattern displayed on the digital micro-mirror device (DMD) orLC-SLM at the Fourier plane can separate the diffraction light coming from different depths into different angular directions being recorded by different portions of the single image plane.
BISCp6-25 Underwater image enhancement algorithm based on granular computing Yingjuan Xie, Xinnan Fan, Haiyan Xu, Zhuo Zhang, Junfeng Chen
Hohai University, China
We proposed an image enhancement algorithm based on granular computing to enhance underwater optical image in this paper. The simulation and experiment results verify the effectiveness of the algorithm.
BISCp6-26 An edge detection method based artificial bee colony for underwater dam crack image Zhang zhuo, Fan xinnan, Xie yingjuan, Xu haiyan
Hohai University, China
In the proposed model, lateral inhibitory network is firstly presented to enhance edge contrast in complex underwater environment.Then, artificial bee colony is improved to optimize target edge.Experimental results show it is efficient and effective.
BISCp6-27 Practical image quality evaluation for whole slide imaging scanner Md Shakhawat Hossain, Toyama Nakamura, Masahiro Yamaguchi
Tokyo Institute of Technology, Japan
We propose a method for evaluation of image quality for whole slide imaging system by eliminating false detection due to tissue artefacts. The method enables more efficient and reliable detection of slides to rescan or to score scanned image.
BISCp6-28 Speckle reconstruction based on oversampling smoothness algorithm Hui Chen, Yesheng Gao, Xingzhao Liu
Shanghai Jiao Tong University, China
Target object image would deteriorate into unrecognizable speckle pattern when encountering with scattering media. In this paper, a method combining correlation method and oversampling smoothness is proposed. It is used for target object reconstruction from scattered speckle pattern.
BISCp6-29 Speckle reconstruction method based on machine learning Hui Chen, Yesheng Gao, Xingzhao Liu
Shanghai Jiao Tong University, China
Scattering media would deteriorate an object image into unrecognizable speckle pattern. Support vector classification and support vector regression is utilized to reconstruct the object image from speckle pattern.
BISCp6-30 Fractality of biospeckle pattern observed in blood coagulation process Naomichi Yokoi1, Yoshihisa Aizu2, Jun Uozumi3
1National Institute of Technology, Asahikawa College, Japan,
2Muroran Institute of Technology, Japan,
3Hokkai-Gakuen University, Japan
It has been known that speckle images observed for living bodies illuminated by laser light sometimes show fractal appearances. In this study, we investigate fractality of biospeckle pattern observed in coagulation process of horse blood.
BISCp6-31 Computational ghost imaging by using complementary illumination patterns Jung-Ping Liu
Feng Chia University, Taiwan
We proposed to use complementary illumination patterns to perform CGI. In addition, we applied Gerchberg-Saxton-like algorithm to optimize the reconstructed image. By this way, the signal-to-noise ratio (SNR) can be significantly reduced.
BISCp6-32 Preliminary study on X-ray phase-contrast imaging with tilted-grid Myung-Joon Kwack, Sooyeul Lee, Seung-hoon Chae
ETRI, Korea
We present preliminary experimental results of X-ray phase-contrast imaging with tilted-grid. Two-dimensional phase gradient information of a PMMA phantom is successfully defined by employing the tilted-grid in a conventional X-ray imaging setup.
BISCp6-34 Implementation of a Raspberry-Pi-based LED array microscope for multi-contrast images Hidenobu Arimoto1, Wataru Watanabe2
1AIST, Japan,
2Ritsumeikan University, Japan
The light emitting diode (LED) array microscope enables various multi-contrast imaging such as bright-field, dark-field and differential phase-contrast by various illumination patterns without any expensive optical components.
BISCp6-35 Image acquisition with smartphone-based LED array microscope Kazuko Koda1, Shu Uenoyama1, Ryo Sugimoto1, Ryoji Maruyama1, Hidenobu Arimoto2, Wataru Watanabe1
1Ritsumeikan University, Japan,
2AIST, Japan
The light emitting diode (LED) array microscope enables various multi-contrast imaging such as bright-field, dark-field and differential phase-contrast (DPC) by various illumination patterns without any expensive optical components.
BISCp6-36 Scan-less, line-filed, confocal phase imaging with dual-comb microscopy Eiji Hase1, 2, Takeo Minamikawa1, 2, Yasuhiro Mizutani2, 3, Tetsuo Iwata2, 4, Hirotsugu Yamamoto2, 5, Takeshi Yasui2, 4
1Tokushima Univ., Japan,
2JST, ERATO MINOSHIMA Intelligent Optical Synthesizer (IOS), Japan,
3Osaka Univ., Japan,
4Tokushima Univ., Japan,
5Utsunomiya Univ., Japan
We constructed the scan-less, line-field, confocal microscopy with the phase-contrast modality by a combination of wavelength-to-space-conversion optical frequency comb with dual-comb spectroscopy, and then demonstrate the proof-of-principle experiment of the scan-less confocal phase line-imaging.
BISCp6-37 Visual search efficiency depending on spatial layout of stimuli in volumetric image T. Pladere, V. Konosonoka, K. Panke, G. Krumina
University of Latvia, Latvia
The spatial layout of stimuli in horizontal and vertical dimension had a bigger impact on visual search performance comparing to the third dimension on a volumetric multi-planar display, also reflected in the subjective difficulty evaluation.
BISCp6-38 Real-time detection of 192Ir gamma-ray source positon using organic scintillator array sensor in HDR brachytherapy Young Beom Song, Bongsoo Lee, Sang Hun Shin
Chung-Ang University, Korea, Republic of
In this study, we fabricated an organic scintillator array sensor (OSAS) based the array of organic scintillators. The scintillator array of OSAS for detecting positions of 192Ir gamma-ray source was fabricated using four types of organic scintillators, which emit the scintillating lights of different wavelength, respectively.
BISCp6-39 Electron beam addressable potentiometric sensor for ion distribution imaging with high resolution Wataru Inami, Yoshimasa Kawata
Shizuoka Univ, Japan
We have developed an electron beam addressable potentiometric sensor to improve the spatial resolution. Ion sensors are widely used in the fields of medical and life science, food and material development, environmental protection and so on. However, the spatial resolution of the ion distribution imaging sensor is limited by the diffraction limit of light or microfabrication technology.
BISCp6-40 Design of add-on optics for optimization of cot endoscope camera for epiduroscopic surgery Khanh Phuong Tran
Intelligent Systems Research Institute, Korea, Republic of
This paper presents a method for designing an add-on lens assembly to optimize the performance of Chip-on-the-Tip (COT) endoscope. In particular, an add-on lens assembly is designed here attributes to a commercially available COT camera, NanEye, in such that provides a FOV of 110º and DOF of 1.5 mm to 8 mm under the refractive index of water.
BISCp6-41 Disturbance location algorithm of the single-core fiber optic sensor based on frequency domain Haiyan Xu, Yingjuan Xie
Hohai Univ, China
A novel distributed fiber-optic sensor based on Wavelength Division Multiplex (WDM) for determining the position of disturbances is presented. Theory analysis and experiment results show that the proposed algorithm can realize the detection and location of the multipoint disturb signals rapidly and effectively.
BISCp6-42 Design optimization of a single-mode microring resonator for label-free detection of biomarkers within a tunable spectral range of 2 nm Prabodh Panindre1, N. S. Susan Mousavi2, Sunil Kumar1, 3
1New York University, USA,
2Institute for Research in Fundamental Sciences, Iran,
3New York University Abu Dhabi, United Arab Emirates
The electromagnetic frequency domain parametric analysis using finite element numerical technique quantifies the effect of geometrical design parameters of microring resonator on its optical characteristics to optimize its performance for label-free detection of nano-biomarkers.
BISCp6-43 Low dose of narrow-band ultraviolet B lamp for improving vitamin D synthesis with minimum skin damage Lin Yu-Hsuan1, Yi-Wen Chiu2, 3, Ming-Yen Lin2, 3, Siao-Ping Tsai2, Feng-Xuan Jian3, Shang-Jyh Hwang2, 3, Kuo-Cheng Huang1
1National Applied Research Laboratories, Taiwan,
2Kaohsiung Medical University Hospital, Taiwan,
3Kaohsiung Medical University, Taiwan
This study proposes that the UV irradiation with a wavelength of 310-320 nm can effectively improve the vitamin D synthesis and minimize the skin damage.
BISCp6-44 Photostability study of CdTe quantum dots using laser induced fluorescence Souad A. Elfeky, A.El-Hussein
The National Institute of Laser Enhanced Science, Cairo University, Egypt
Semiconductor quantum dots (QDs) having high quantum yields and unique photostability. This research studies the optical properties of the synthesized CdTe QDs with two different sizes using Laser-induced fluorescence (LIF) for investigating their photostability. 
BISCp6-45 Edge contrast enhancement at multiple planes using Forked Shaped defocus grating Sunil Vyas, Chen Yen Lin, Yuan Luo
National Taiwan Univ, Taiwan
We report on the implementation of spiral phase contrast imaging at multiple planes using forked-shaped defocus grating. The dual function of grating helps in simultaneous generation of multiple edge enhanced images corresponding to different depths.
BISCp6-46 Orthogonal functional system for finite Fresnel transform Tomohiro Aoyagi, Kouichi Ohtsubo, Nobuo Aoyagi
Toyo University, Japan
The Fresnel transform has been studied mathematically and revealed the topological properties in Hilbert space. Main aim is to reveal the property of band-limited function. We seek the function that its total power is maximized in finite Fresnel transform plane, on condition that an input signal is zero outside the bounded region.
BISCp6-47 Nanoscale three-dimensional imaging of biological tissue with X-ray holographic tomography Alexandra Pacureanu, Julio Caesar da Silva, Yang Yang, Sylvain Bohic, Peter Cloetens
European Synchrotron Radiation Facility, France
Enabling exploration of biological tissue in three-dimensions at sub-cellular scale is instrumental for advancing our understanding of biological systems and for finding better ways to cope with diseases. Over the last few years, remarkable advances in microscopy facilitated probing cells and tissues at the nanometer scale but many limitations are yet to be overcome. Here we present a novel technique which enables label-free volume imaging of biological tissue with pixel sizes down to 25 nm while maintaining extensive sample coverage. X-ray holographic nanotomography is a full-field 3D imaging technique which benefits from the deep penetration of X-rays and the powerful mechanism of phase contrast. By using cryogenic sample preservation, the tissue can be investigated close to the native state. The unprecedented data created by this technique opens new avenues in life sciences research.

BISC7: Optical Diagnosis and Treatment
Chair: Joseph Rosen(Ben-Gurion Univ of the Negev, Israel)

Room 419

Start End Session No. Title Speaker Description
15:00 15:15 BISC7-1 Quantitative evaluation of healing degree in injured tendons based on orientation analysis of collagen fibers by using Fourier-transform second-harmonic-generation microscopy and its relationship to mechanical property Eiji Hase1, Takeo Minamikawa2, Katsuya Sato2, Daisuke Yonekura2, Mitsuhiko Takahashi3, Takeshi Yasui2
1Japan Sychrotron Radiation Reasearch Institute, Japan,
2Tokushima Univ., Japan,
3Tokushima Pref. Cent. Hosp., Japan
We used Fourier-transform second-harmonic-generation (FT-SHG) microscopy to analyze the orientation of collagen fibers in healing rabbit tendons recovered from an artificial transection and assessed the correlation between the orientation parameter and Young’s modulus.
15:15 15:30 BISC7-2 Noninvasive estimation of light scattering and hemoglobin concentration in mice cutaneous carcinogenesis through multispectral imaging Izumi Nishidate1, Satoko Kawauchi2, Shunichi Sato2
1Tokyo Univ of Agriculture and Technology, Japan,
2National Defense Medical College Research Institute, Japan
The proposed multispectral diffuse reflectance images acquired at isosbestic wavelengths of hemoglobin were able to estimate the total hemoglobin concentration and tissue scattering parameter of mice skin during cutaneous two-stage chemical carcinogenesis.
15:30 15:45 BISC7-3 Characterization of cancer metastasis in model mice by multiphoton microscopy and Raman Spectroscopy Yusuke Oshima, Shigehiro Koga, Yuji Watanabe
Ehime Univ, Japan
In this study, we investigate molecular dynamics in both cancer cells and their environment in xenograft models and spontaneous metastasis models using Raman spectroscopy and nonlinear optical imaging. We are also constructing a custom-designed Raman spectral imaging system to reveal the metastasis process and to evaluate therapeutics toward the clinical application of the technique.
15:45 16:00 BISC7-4 Glucose sensing in the presence of scattering particles using decomposition of partial Mueller matrix Pradipta Mukherjee, Yukitoshi Otani
Utsunomiya Univ, Japan
A Partial Mueller matrix polarimeter retrieves a subset of sample polarization properties that can be useful for specific measurement. A partial Mueller matrix decomposition method is proposed to retrieve the optical rotation and depolarization simultaneously for measuring glucose concentration in the presence of scattering. A dual-photoelastic-modulator based Mueller matrix polarimeter is designed for this purpose. We verify the proposed decomposition method by measuring different glucose concentrations mixed with scattering particles.
16:00 16:15 BISC7-5 Quantitative in situ time-series evaluation of osteoblastic collagen synthesis under cyclic strain using second-harmonic-generation microscopy Katsuya Sato1, Eiji Hase2, Takeo Minamikawa1, Takeshi Yasui1
1Tokushima University, Japan,
2Japan synchrotron radiation research institute, Japan
The aim of this study is to provide an in situ method to non-invasively monitor osteoblastic collagen synthesis under mechanical stimulation. We applied second-harmonic-generation (SHG) microscopy to monitor the collagen fibers produced by osteoblast-like cells. To evaluate the influence of mechanical stimulation on collagen synthesis and maturation, we compared SHG images of osteoblast-produced collagen fibers with and without a cyclic stretch stimulus. We acquired SHG images every 7 days for 3 weeks at different stimulus conditions. Image analysis of the average SHG intensity indicated that the amount of osteoblastic collagen synthesis was significantly enhanced by the cyclic stretch compared with the the non-stretched condition, while there was no significant difference between the two stimulus conditions. Furthermore, the maturity of the collagen fibers was not affected in the early stage of bone formation by the mechanical stimulus.
16:15 16:30 BISC7-6 Fluid dynamic modeling and comparison of the intraocular pressure changes in eyes with SMILE and LASIK Kuo-Jen Wang1, Cheliang Tsai1, I-Jong Wang2
1Crystalvue Medical Corp, Taiwan,
2National Taiwan University Hospital, Taiwan
We developed an intraocular pressure analytic model utilizing fluid dynamics, solid mechanics, and ray-tracing technique to simulate the air-puff noncontact tonometry for post-SMILE and post-LASIK IOP measurement.
16:30 16:45 BISC7-7 Enhanced therapeutic effect of an antitumor agent on malignant glioma in rats by photomechanical wave-based transvascular drug delivery Yumiko Koshi1, Izumi Nishidate1, Shunichi Sato2
1Tokyo University of Agriculture and Technology, Japan,
2National Defense Medical College Research Institute, Japan
We used a photomechanical wave (PMW) to enhance the delivery efficiency of an antitumor drug, temozolomide (TMZ) in a F98 rat glioma model, and showed that therapeutic effect of TMZ was improved by PMW application.
16:45 17:00 Closing Remark &
Award Ceremony
Osamu Matoba
Kobe University, Japan