The 4th Biomedical Imaging and Sensing Conference 2018(BISC2018)

Program

April 25 Wed.

OMC&BISC1: OMC and BISC Joint Session 1
Chair: Takashige Omatsu(Chiba University, Japan)

Room 418

Start	End	Session No.	Title	Speaker	Description
13:30	14:00	OMC&BISC1-1Plenary	High Temporal and Spatial Pattern Stimulation to Manipulate Brain Function	Hiroaki Wake¹, Koichiro Haruwaka¹, Xiangyu Quan², Osamu Matoba² ¹Kobe University of Graduate School of Medicine, Japan, ²Kobe University, Japan	In the central nervous system (CNS), glial cells, originally termed “nervenkitt,” recently focused because of the understanding of their physiological functions. Here, we focused how glial cell regulates the function of neuronal circuits using in vivo two photon microscope.
14:00	14:30	OMC&BISC1-2Plenary	Femtosecond Laser Trapping, Assembling, and Ejection Dynamics of Dielectric Nanoparticles in Solution	Hiroshi Masuhara¹, J. Chen¹, W. Chiang^{1, 2}, A. Usman³, T. Sugiyama^{1, 4}, J. Hofkens² ¹National Chiao Tung University, Taiwan, ²Katholieke Universiteit Leuven, Belgium, ³Universiti Brunei Darussalam, Brunei Darussalam, ⁴Nara Institute of Science and Technology, Japan	We study femtosecond laser trapping dynamics of Rayleigh particles by examining polystyrene, silica, and surfacemodified silica nanoparticles with different diameter and by changing solvent viscosity.
14:30	15:00	OMC&BISC1-3Plenary	Monitoring Ruffling Cells by Lattice Light-sheet Microscopy	Bi-Chang Chen, Wei-Chun Tang, Peilin Chen Academia Sinica, Taiwan	The membrane ruffling can be triggered by rapamycin and/or blue light. After stimulation, the three- dimensional dynamics of membrane ruffling has been recorded by lattice light-sheet microscope (LLSM), which is capable of high spatial and temporal recording over three-dimensions.
15:00	15:30	Break

OMC&BISC2: OMC and BISC Joint Session 2
Chair: Osamu Matoba(Kobe University, Japan)

Room 418

Start	End	Session No.	Title	Speaker	Description
15:30	15:45	OMC&BISC2-1Invited	Computational Imaging and Reconstruction in Digital Holographic Microscopy	Edmund Y.M. Lam University of Hong Kong, Hong Kong	Digital holographic microscopy captures the 3D information of biological specimens as holograms, which can then be reconstructed into sectional images computationally. In this talk, we will discuss recent advances in such algorithms and applications.
15:45	16:00	OMC&BISC2-2	Observation of Immunostained Microtubules Using Three-Dimensional Superresolution Microscope with Two-Color Annular Wave Plate	Yoshinori Iketaki¹, B. Nandor², D. Okada³, H. Kumagai⁴ ¹Olympus Corp, Japan, ²Budapest University of Technology and Economics, Hungary, ³Kitasato University, School of Medicine, Japan, ⁴Kitasato University, School of Allied Health Sciences Physics, Japan	Three-dimensional super-resolution microscopy based on fluorescence depletion (3D-SRM) was applied to the observation of immunostained microtubules having complicated structures stacking each other.
16:00	16:15	OMC&BISC2-3	Optical Trapping of Quantum-Dot Conjugated AMPA-type Receptors Depened on Initial Assembling States	Tatsunori Kishimoto^{1, 2}, Y. Maezawa¹, S. N. Kudoh², T. Taguchi³, C. Hosokawa^{1, 2, 4} ¹National Institute of Advanced Industrial Science and Technology (AIST), Japan, ²Kwansei Gakuin University, Japan, ³National Institute of Information and Communications Technology (NICT), Japan, ⁴Advanced Photonics and Biosensing Open Innovation Laboratory, AIST-Osaka University, Japan	AMPA-type glutamate receptor (AMPAR) is one of the major neurotransmitter receptors at excitatory synapses.
16:15	16:30	OMC&BISC2-4	Enhanced Collection Efficiency of Vesicles in A Suspension by Optical Pressure Using Gold Nanoparticles	Takashi Kaneta, M. Kuboi, N. Takeyasu Okayama University, Japan	We describe a collection of micro-vesicles on a glass substrate using the optical pressure of a laser beam. The laser beam was focused on the glass substrate which sandwiches a suspension containing micrometer-sized vesicles prepared by a phospholipid.
16:30	16:45	OMC&BISC2-5	Rotational Dynamics of Bacteria in An Optical Tweezer	Sharath Ananthamurthy University of Hyderabad, India	The swimming of bacterium in fluid occurs in low-Reynold’s regime. The trapping of a bacterium using laser light gives us information about the hydrodynamic coefficients which are very important in understanding the efficiency of swimming of bacterium.

April 26 Thu.

BISC1: Advanced Optical Miroscopy 1
Chair: Toyohiko Yatagai(Utsunomiya University, Japan)

Room 419

Start	End	Session No.	Title	Speaker	Description
9:30	9:45		Opening Remarks	Toyohiko Yatagai Utsunomiya University, Japan
9:45	10:15	BISC1-1 Invited	Coherent brightfield (COBRI) micros copy for ultrahig h-speed single- particle tracking on lipid bilayer membranes	Yi-Hung Liao, Chia-Lung Hsie Academia Sinica, Taiwan	Detecting linear scattering light from biological entity is useful for label-free bioimaging. Coherent brightfield (COBRI) microscopy provides the sensitivity to see individual native biological nanoparticles in live cells with nanometer spatial localization precision and microsecond temporal resolution.
10:15	10:45	BISC1-2 Invited	Visualizing a beating zebrafish heart with improved line-scan microscopy	Nanguang Chen National Univ of Singapore, Singapore	We have developed a high-speed line-scan optical microscope that is capable of acquiring high-resolution, high-contrast fluorescence images at more than 100 fps. This advanced imaging technique has been applied to multi-dimensional imaging of zebrafish heart.
10:45	11:00	Break
11:00	11:30	BISC1-3 Invited	Fast, long-term super-resolution imaging with Hessian structured illumination microscopy	Xiaoshuai Huang¹, Junchao Fan², Liuju Li¹, Haosen Liu², Runlong Wu³, Yi Wu⁴, Lisi Wei¹, Heng Mao⁵, Amit Lal⁶, Peng Xi⁶, Liqiang Tang⁷, Yunfeng Zhang³, Yanmei Liu¹, Shan Tan², Liangyi Chen¹ ¹Institute of Molecular Medicine, Peking University, China, ²Huazhong University of Science and Technology, China, ³School of Electronics Engineering and Computer Science, Peking University, China, ⁴School of Software and Microelectronics, Peking University, China, ⁵School of Mathematical Sciences, Peking University, China, ⁶Department of Biomedical Engineering, Peking University, China, ⁷ColdSpring Science Corporation, China	To increase the temporal resolution and maximal imaging time of super-resolution (SR) microscopy, we have developed a deconvolution algorithm for structured illumination microscopy based on Hessian matrixes (Hessian-SIM).
11:30	11:45	BISC1-4	Multi-focal holographic slit confocal fluorescence microscopy	Hung-Chun Wang¹, Yuan Luo², Chou-Min Chia² ¹National Tsing Hua University, Taiwan, ²National Taiwan University, Taiwan	The non-axial line-scanning multifocal confocal microscopy incorporates multiplex volume holographic grating (MVHG) in illumination and combination of multifocal image system to image multiplane at the same time. The system resolve volume tissue fast and well, offering a solution of replacing biopsy.
11:45	12:00	BISC1-5	Lateral spatial resolution improvement in laser scanning fluorescence microscopy using a subdiffraction limit optical spo	Takahiro Nishimura¹, Yusuke Ogura¹, Yosuke Tamada², Jun Tanida¹ ¹Osaka Univ, Japan, ²NIBB, Japan	This paper confirmed that use of a computer generated hologram that makes the size of the generated spots smaller than the single diffraction limited can improve the lateral spatial resolution of the laser scanning fluorescence microscopy.
12:00	13:15	Lunch

BISC2: Optical Coherence Tomography
Chair: Yuan Luo(National Taiwan University, Taiwan)

Room 419

Start	End	Session No.	Title	Speaker	Description
13:15	13:45	BISC2-1 Invited	Endoscopic optical coherence tomography and angiography for gastroenterology applications	Hsiang-Chieh Lee^{1, 2}, Kaicheng Liang², Osman O. Ahsen², Zhao Wang², Marisa Figueiredo³, Benjamin Potsaid^{2, 4}, Vijaysekhar Jayaraman⁵, Qin Huang³, Hiroshi Mashimo^{3, 6}, James G. Fujimoto² ¹National Taiwan Univ, Taiwan, ²Massachusetts Institute of Technology, USA, ³VA Boston Healthcare System, USA, ⁴Thorlabs Inc., USA, ⁵Praevium Research Inc., USA, ⁶Harvard Medical School, USA	Endoscopic optical coherence tomography (OCT) angiography enables volumetric coregistered architectural and microvasculature imaging of the human gastrointestinal tract in vivo. In this talk, we will discuss technical advances and clinical gastroenterology applications with the endoscopic OCT angiography technique.
13:45	14:00	BISC2-2	Evaluation of photodamage with polarization-sensitive optical coherence tomography	Trung Nguyen Hoang, Cheng Yu Lee, Meng-Tsan Tsai Chang Gung University, Taiwan	Polarization sensitive optical coherence tomography (PS-OCT) is an extension of conventional optical coherence tomography (OCT) which enable the function to investigate birefringence characteristic of materials of biological tissue.
14:00	14:15	BISC2-3	A 2.8-mm beam diameter system for retinal imaging with OCT and adaptive optics	Maddipatla Reddikumar, Joel Cervantes, Yukitoshi Otani, Barry Cense Utsunomiya Univ, Taiwan	A less-complex 2.8-mm beam diameter spectral domain optical coherence tomography system with an adaptive optics module presented. In this system a Shack-Hartmann wavefront sensor used for aberration sensing and the Deformable mirror used for aberration correction.
14:15	14:30	BISC2-4	Study on photothermolysis with multi-functional, high-resolution optical coherence tomography	Tai-Ang Wang¹, Ming-Che Chan¹, Meng-Tsan Tsai² ¹National Chiao Tung University, Taiwan, ²Chang Gung University, Taiwan	A high-resolution OCT is developed for observation of photothemolysis effect. Using a compact supercontinuum generation laser to achieve high resolution, our HROCT is centered at 750nm, with 300nm bandwidth, and axial resolution < 1 micron.
14:30	14:45	BISC2-5	Motion artifacts in endoscopic catheter-based images: simulation and motion correction method	Elham Abouei¹, Anthony M. D. Lee², Geoffrey Hohert², Michelle C. Cua², Pierre Lane² ¹Univ of British Columbia, Canada, ²BC cancer research center, Canada	A model of motion artifacts for 3D/2D rotational pullback catheter data and a motion correction method called azimuthal en face image registration is presented. Qualitative and quantitative evaluations of the method are analysed for OCT-AFI images.
14:45	15:00	BISC2-6	Assessment of micro-optical coherence tomography (μOCT) as an imaging tool for pancreatic disease diagnosis	Chi Hu¹, Linbo Liu² ¹Northwestern Polytechnical University, China, ²Nanyang Technological University, Singapore	The feasibility of µOCT as an imaging tool for pancreatic disease diagnosis is evaluated.Ex vivo experiments with both normal tissues and those with pancreatic edema are conducted to verify the applicability of µOCT
15:00	15:30	Break

BISC3: Optical Imaging of Multimodal and Biomedical Information
Chair: Tatsuki Tahara(Kansai Univ., Japan)

Room 419

Start	End	Session No.	Title	Speaker	Description
15:30	16:00	BISC3-1 Invited	Spatial resolution enhancement in laser scanning microscopy using vector beams	Yuichi Kozawa^{1, 2}, Shunichi Sato¹ ¹Tohoku Univ, Japan, ²JST, PRESTO, Japan	Cylindrical vector beams have unique features such as the formation of a small focal spot under tight focusing condition. We utilize such characteristic to enhance the spatial resolution in laser scanning microscopy.
16:00	16:30	BISC3-2 Invited	Computational imaging utilizing volume hologram	Tomoya Nakamura, Masahiro Yamaguchi Tokyo Institute of Technology, Japan	Volume hologram is a powerful tool for enabling computational imaging. This report mainly introduces the light-field imaging system utilizing volume hologram, and the talk corresponding to this report also addresses other applications of volume hologram
16:30	17:00	BISC3-3 Invited	Multimodal digital holographic microscopy for simultaneous phase and fluorescence imaging	Xiangyu Quan¹, Osamu Matoba¹, Yasuhiro Awatsuji² ¹Kobe Univ., Japan, ²Kyoto Inst. of Tech., Japan	This paper introduces a new type of multimodal digital holographic microscopy for biological applications. Off-axis digital holography is applied both in 3D phase imaging and 3D fluorescence imaging. In addition, image recovery by iterative method to obtain focused fluorescence images are introduced.
17:00	17:30	BISC3-4 Invited	Fluorescent molecular force probes for rheology and mechanobiology	Shohei Saito Kyoto Univ., Japan	Fluorescent molecular force probes have been developed for rheology and mechanobiology. To realize fluorescence response to pico-Newton forces, we have explored flexible force probes based on a conformational change of flexible molecules.
17:30	18:00	BISC3-5 Invited	Illuminating gene expression dynamics by optogenetics	Akihiro Isomura Kyoto Univ., Japan	Cells receive diverse signaling cues from their environment that trigger cascades of biochemical reactions in a dynamic manner. Live-cell imaging technologies have revealed dynamic patterns of gene activities; however it has been challenging to clarify….

April 27 Fri.

BISC4: Multimodal Imaging and Devices
Chair: Yoshihisa Aizu(Muroran Institute of Technology, Japan)

Room 419

Start	End	Session No.	Title	Speaker	Description
9:00	9:15	BISC4-1	Non-scanning in-vivo three-dimensional hybrid structured illumination microscopy h-speed single- particle tracking on lipid bilayer membranes	Ju-Hsuan Chien National Taiwan University, Taiwan	Demonstrate HiLo structured illumination microscopy by the use of digital micro-mirror device (DMD) and the focal tunable lens (FTL). This system are high-resolution, wide-field optically sectioning and viewing in vivo biological tissue samples without mechanical scanning.
9:15	9:30	BISC4-2	Shape based pharmacokinetic fluorescence optical tomography	Omprakash Gottam, Naren Naik Indian Institute of Technology Kanpur, India	Spatially varying pharmacokinetic rates in a tissue help in identifying abnormal regions since they govern the exchange of fluorophore dye between blood plasma and tissue.
9:30	9:45	BISC4-3	Fast spatial domain reconstruction for structured illumination microscopy	Xing Zhou, Dan Dan, Baoli Yao Xi’an Institute of Optics and Precision Mechanics, China	As a wide-field super-resolution (SR) technique, structured illumination microscopy (SIM) features the merits of fast imaging speed, low excitation intensity and a large field of view.
9:45	10:00	BISC4-4	Visible resonance Raman spectroscopy detect key molecular biomarker vibrations to characterize for human brain gliomas	Cheng-Hui Liu¹, Yan Zhou², Binlin Wu³, Xinguang Yu⁴, Gangge Cheng², Chunyuan Zhang¹, Cuicui Lu⁵, Ke Zhu⁶, Robert R. Alfano¹ ¹Institute for Ultrafast Spectroscopy and Lasers, USA, ²Air Force General Hospital, China, ³Southern Connecticut State University, USA, ⁴PLA General Hospital, China, ⁵Qian xuesen Laboratory of Space Technology, China, ⁶Institute of Physics, Chinese Academy of Sciences (CAS), China	Key Raman vibrational modes at 1129cm^-1 and 1338cm^-1 were observed to characterize gliomas using WITec300R visible resonance Raman (VRR) spectrometer. This work may aid neurosurgeons better decide surgical margins of cancers.
10:00	10:15	BISC4-5	Long-life plastic optical fiber probes for scanning near-field optical microscope	Anton Smirnov, Giovanni Dietler, Sergey Sekatskii Ecole Polytechnique Fédérale de Lausanne, Switzerland	Sharpened glass fiber SNOM probes have certain severe drawbacks. We are presenting alternative SNOM probes made from plastic optical fibers. These probes demonstrate an excellent performance in both topographical and optical channels after intense use.
10:15	10:30	BISC4-6	Effect of speckle pattern illumination on holographic recording and reconstructio	Vinu R. V., Darshika Singh Rakesh Kumar Singh, IIST, India	A speckle field illumination technique for recording and reconstruction of the complete wavefront is proposed and experimentally demonstrated and compare with conventional holographic approach. This technique is expected to play an important role in studying the polarization sensitive materials.
10:30	10:45	BISC4-7	Isotropic quantitative differential phase contrast microscopic imaging	Yu-Zi Lin, Kuang-Yuh Huang National Taiwan University, Taiwan	We propose a new illumination method to achieve isotropic differential phase contrast microscopic imaging efficiently. Recover quantitative phase image of thin transparent sample under 2-axis intensity measurements. Improve the accuracy and stability of phase recovery in conventional differential phase contrast microscopy.
10:45	11:15	Break

BISC5: Digital Holographic Microscopy
Chair: Wataru Watanabe(Ritsumeikan University)

Room 419

Start	End	Session No.	Title	Speaker	Description
11:15	11:45	BISC5-1 Invited	Incoherent digital holography for biomedical imagin	Joseph Rosen Ben-Gurion Univ of the Negev, Israel	The evolution of the FINCH incoherent digital holography method is described. Following the review of FINCH, other recently developed self-reference single-channel incoherent hologram recorders, branched out from FINCH, are discussed and several biomedical-related applications are described.
11:45	12:00	BISC5-2	Investigations of wavelength resolution and adoptable phase shifts in phase-shifting color digital holography with 2pi ambiguity and wavelength-multiplexed images	Tatsuki Tahara^{1, 2}, Reo Otani³, Yasuhiko Arai¹, Yasuhiro Takaki⁴ ¹Kansai Univ, Japan, ²PRESTO, Japan Science and Technology Agency, Japan, ³SIGMAKOKI CO. LTD., Japan, ⁴Kansai Univ, Japan	We investigate wavelength resolution and adoptable phase shifts in phase-shifting color digital holography with 2pi ambiguity and wavelength-multiplexed images.
12:00	12:15	BISC5-3	Single-shot incoherent digital holography using parallel phase-shifting radial shearing interferometry	Syogo Mochida¹, Takahito Fukuda¹, Yasuhiro Awatsuji¹, Kenzo Nishio¹, Osamu Matoba² ¹Kyoto Institute of Technology, Japan, ²Kobe University, Japan	We propose single-shot incoherent digital holography using parallel phase-shifting radial shearing interferometry. We applied this technique to the three-dimensional measurement of objects illuminated by incoherent light and put on different depth positions.
12:15	13:00	Lunch

BISCp6: Poster Session

Exhibition Hall A

Start	End	Session No.	Title	Speaker	Description
13:00	14:30	BISCp6-1	Non-invasive glucose monitoring based on optical coherent tomography	Quoc-Hung Phan1Tseng-Lin Chen¹, Yu-Lung Lo^{1, 2}, ¹Department of Mechanical Engineering, National Cheng Kung University, Taiwan, ²Advanced Optoelectronic Technology Center, National Cheng Kung University, Taiwan	A novel technique based on optical coherence tomography (OCT) for noninvasive glucose monitoring is proposed. The feasibility of the proposed technique is demonstrated by detecting the glucose concentration of aqueous solution ranging from 0~4000 mg/dL with 0.02% lipofundin.
		BISCp6-2	Monitoring protein-related degeneration of Drosophila eyes with optical coherence tomography	Chia-Heng Wu¹, Meng-Tsan Tsai² ¹Chang Gung Univ, Taiwan, ²Department of Electrical Engineering, Chang Gung University, Taiwan	In this study, we propose to use optical coherence tomography (OCT) for the study of protein-related degeneration of Drosophila eyes. With OCT, the bristles of Drosophila eye can be identified and different mutant Drosophila were scanned with OCT for investigation of progress of protein-related degeneration.
		BISCp6-3	Parallel phase-shifting radial shearing interferometry and its numerical verification	Syogo Mochida¹, Takahito Fukuda¹, Yasuhiro Awatsuji², Kenzo Nishio³, Osamu Matoba⁴ ¹Department of Electronics, Graduate School of Science and Technology, Kyoto Institute of Technology, Japan, ²Faculty of Electrical and Electronics, Kyoto Institute of Technology, Japan, ³Advanced Technology Center, Kyoto Institute of Technology, Japan, ⁴Department of Systems Science, Graduate School of System Informatics, Kobe University, Japan	We propose parallel phase-shifting radial shearing interferometry for single-shot measurement of wavefront from an object. We numerically simulated and demonstrated the wavefront measurement of two particles assumed as object wave by using the proposed technique.
		BISCp6-4	Motion-picture phase imaging by an integrated optical system of a parallel phase-shifting digital holographic microscope	Kazuki Shimizu¹, Takahito Fukuda¹, Peng Xia², Yasuhiro Awatsuji³, Kenzo Nishio⁴, Osamu Matoba⁵ ¹Department of Electronics, Graduate School of Science and Technology, Kyoto Institute of Technology, Japan, ²National Institute of Advanced Industrial Science and Technology, Japan, ³Kyoto Institute of Technology, Faculty of Electrical Engineering and Electronics, Japan, ⁴Advanced Technology Center, Kyoto Institute of Technology, Japan, ⁵Department of Systems Science, Graduate School of System Informatics, Kobe University, Japan	The authors have designed and constructed an integrated optical system of parallel phase-shifting digital holographic microscope. Motion-picture phase imaging of a dynamic minute transparent specimen at 1,000 fps has been demonstrated by the microscope.
		BISCp6-5	Digital holographic microscopy using speckle illuminations and two-wavelength method	Hideki Funamizu¹, Jun Uozumi², Yoshihisa Aizu³ ¹Yusei Onodera, Muroran Institute of Technology, Japan, ²Faculty of Engineering, Hokkai-Gakuen University, Japan, ³Muroran Institute of Technology, Japan	It has been known that spatial resolution of digital holographic microscopy (DHM) can be enhanced using speckle illuminations. In this study, we report the application of the two-wavelength method to DHM using speckle illuminations.
		BISCp6-6	Tomographic phase imaging of RBCs in blood coagulation structures using digital holographic microscopy	Hideki Funamizu, Ryoji Goto, Yoshihisa Aizu Muroran Institute of Technology, Japan	Blood coagulation is an important role in hemostasis. In this study, to observe the degree of blood coagulation, we demonstrate a tomographic phase imaging of aggregation structures of RBCs using digital holographic microscopy.
		BISCp6-7	Reconstruction of complex amplitude by lensless phase-shift digital holography through an opaque glass plate	Akinori Igarashi, Wataru Watanabe Ritsumeikan University, Japan	Optical imaging through diffusive or scattering media has attracted much attention. Digital holographic microscopy provides quantitative phase imaging thorough diffusive media. We experimentally reconstruct intensity and phase images of an object through an opaque ground glass screen by means of digital holography.
		BISCp6-8	Digital holographic size measurement of Daphnia pulex	Kota Sunayama¹, Hitoshi Miyakawa², Yoshio Hayasak² ¹Center for Optical Research and Education (CORE), Utsunomiya University, Japan, ²Center for Bioscience Research and Education (CBRE), Utsunomiya University, Japan	Toxicity of chemical substances should be determined for protecting biological environment. A Daphnia pulex is one of the indicator organisms for searching the toxicity, because the shape is changed depending on the toxicity. Conventional method for its observation has been performed under suppression of its movement in a small thin room with an ordinary optical microscope.
		BISCp6-9	Incoherent holographic imaging of subsurface structures with volume holographic gratings	Yu-Hsin Chia¹, Hung-Chun Wang², Yuan Luo³ ¹Institute of Medical Device and Imaging, National Taiwan University, Taiwan, ²Department of Power Mechanical Engineering, National Tsing Hua University, Taiwan, ³Institute of Medical Device and Imaging, National Taiwan University, Taiwan	Under broadband illumination, a multiplane microscopy incorporating volume holographic gratings (VHGs) to observe three-dimensional structures of biological samples is presented. Here, we experimentally demonstrate this microscopic imaging capability to obtain multiple depth-resolved images of fine structures from eight depths in one shot.
		BISCp6-10	In vivo time-series monitoring of dermal collagen fiber during skin burn healing using second-harmonic-generation microscopy	Eiji Hase^{1, 2}, Ryosuke Tanaka³, Shu-ichiro Fukushima³, Takeshi Yasui^{1, 3} ¹Tokushima University, Japan, ²JASRI/SPring-8, Japan, ³Osaka University, Japan	We applied second-harmonic-generation microscopy for in vivo imaging of healing process in animal skin burn and visualized decomposition, production, and growth of renewal collagen fibers as a series of time-lapse images in the same subject.
		BISCp6-11	In vivo visualization of dermal collagen fibers in human skin using a photonic-crystal-fiber-coupled, hand-held second-harmonic-generation microscope	Yuki Ogura, Kosuke Atsuta, Eiji Hase, Takeo Minamikawa, Takeshi Yasui Tokushima Univ., Japan	We constructed a hand-held second-harmonic-generation microscope for in vivo monitoring of collagen fibers in human skin by fiber delivery of ultrashort pulse light in a large-mode-area photonic-crystal-fiber and a compact microscopy setup.
		BISCp6-12	Analysis of collagen fiber orientation in biological tissues using polarization-resolved second-harmonic-generation microscopy	Takuya Sakaue¹, Eiji Hase², Takeo Minamikawa³ ¹Tokushima Univ, Japan, ²Tokushima Univ, Japan, SPring-8(JASRI), Japan, ³Takeshi Yasui, Tokushima Univ, Japan	We constructed continuously-polarization-resolved SHG microscopy based on rapid polarization rotation every 15 degrees with electric-optic Pockells cell, and applied it for the quantitative analysis of collagen fiber orientation in biological tissues.
		BISCp6-13	In situ monitoring of incised wound healing in animal model using second-harmonic-generation and third-harmonic-generation microscopy	Shu-ichiro Fukushima¹, Yuji Tanaka¹, Eiji Hase^{2, 3}, Kazuma Takeichi², Takeshi Yasui^{1, 2} ¹Osaka University, Japan, ²Tokushima University, Japan, ³JASRI/SPring-8, Japan	We apply the combination of second-harmonic-generation microscopy with third-harmonic-generation microscopy for incised wound animal model, and visualize collagen dynamics during the wound healing process in time series in situ.
		BISCp6-14	Resonance Raman and fluorescence spectroscopy to evaluate increased brain kynurenine pathway activity in samples from patients with Alzheimer’s	Laura A. Sordillo, Lin Zhang, Lingyan Shi, Peter Sordillo, Robert Alfano City College of New York, United States	Resonance Raman and fluorescence spectroscopy were used to assess increased kynurenine pathway activity in brain samples from Alzheimer’s patients and age-matched controls. Increased activity was seen in areas of the brain involved in Alzheimer’s disease.
		BISCp6-15	Development of dual-wavelength microscopic laser speckle contrast imaging system	Cheng-Yu Lee¹, Meng-Tsan Tsai^{1, 2} ¹Chang Gung University, Taiwan, ²Chang Gung Memorial Hospital, Taiwan	Laser Speckle Contrast Imaging (LSCI), which used coherent light, has fully been used for observing blood flow due to its non-invasive, non-contact acquisition method. Generally, LSCI system uses just a single wavelength for measurement. In this research, first, considering the biological characteristics of different reflection rates and absorption, we use two lasers at 633nm and 855 nm and two CCD cameras to build a microscopic LSCI system.
		BISCp6-16	Polarization-resolved second-harmonic-generation imaging of dermal collagen fiber in pre-wrinkled skin of ultraviolet-B-exposed mouse	Shu-ichiro Fukushima¹, Makoto Yonetsu¹, Eiji Hase^{2, 3}, Takeshi Yasui^{1, 2} ¹Osaka University, Japan, ²Tokushima University, Japan, ³JASRI/SPring-8, Japan	We investigate orientation change of dermal collagen fiber in pre-wrinkled skin of ultraviolet-B-exposed mouse skin using polarization-resolved second-harmonic-generation microscopy, and confirm that change of collagen fiber orientation is a trigger of wrinkling in photo-aged skin.
		BISCp6-17	Effects of nitric oxide on cortical hemodynamic responses in the rat brain exposed to a shock wave	Masaki Inaba¹, Shunichi Sato², Izumi Nishidate3 ¹Tokyo University of Agriculture and Technology, Japan, ²National Defense Medical College Research Institute, Japan, ³Tokyo University of Agriculture and Technology, Japan	We examied how the nitric oxide synthesis inhibition altered hemodynamic responses to a laser-induced shock wave(LISW) by diffuse reflectance spectroscopy. The results suggested that hemodynamic abnormalities, or transient hyperemia/hyperoxemia followed by persistent oligemia/hypoxemia, caused by an LISW in the rat cortex was associated with an increased nitric oxide production and its vasodilatory/vasoconstrictory effects.
		BISCp6-18	Partial optical path length in the scalp in subject-specific head models for multi-distance probe configuration of near infrared spectroscopy	Taku Yanagisawa¹, Hiroshi Kawaguchi², Eiji Okada³ ¹Department of Electronics and Electrical Engineering,Keio University, Japan, ²National Institute of Advanced Industrial Science and Technology, Japan, ³Department of Electronics and Electrical Engineering,Keio University, Japan	Light propagation in 45 subject specific head models was calculated to predict the spatial variability of the partial optical path length in the scalp and the weighting factor for the multi-distance probe configuration of NIRS.
		BISCp6-19	Measurement of head phantom by functional near infrared imaging using multi-distance probe configuration	Koki Hayabusa, Eiji Okada Department of Electronics and Electrical Engineering, Keio University, Japan	The topographic image of the head phantom including the local absorption change in gray matter and global absorption change in the scalp was measured by functional near infrared imaging system using multi-distance probe configuration.
		BISCp6-20	Diffuse light reflectometry for measuring scattering and absorption coefficients of a biological tissue	Daiki Maeda, Toshiaki Iwai Tokyo University of Agriculture and Technology, Japan	The optical properties, a scattering and an absorption coefficients, of biological tissues will be used to estimate quantitatively change in bioactivity. Diffuse light reflectometry has been investigated to measure the optical properties of biological tissues from the viewpoints of applicability and practicality.
		BISCp6-21	Adjoint based Hessian evaluation for SPN modeled optical tomography	Nishigandha Patil, Naren Naik Indian Institute of Technology Kanpur, India	We present for the first time an adjoint based evaluation of the Hessian matrix for the SPN-approximation modeled forward operator in optical tomography. The Hessians so calculated are numerically validated with respect to finite difference calculations.
		BISCp6-22	Three-dimensional beam focusing control for lensless vascular endoscopes using local wavefront control	Masaki Hisaka Osaka Electro-Communication Univ, Japan	The focusing beam generated by an optical fiber bundle having locally optical delay has been investigated to simplify the structure of vascular endoscopes. We have demonstrated the beam focusing and scanning using a liquid lens.
		BISCp6-23	Application of scan-less two-dimensional confocal microscopy achieved by a combination of confocal slit with wavelength/space conversion	Eiji Hase^{1, 2}, Takeo Minamikawa^{1, 2}, Yasuhiro Mizutani^{2, 3}, Tetsuo Iwata^{2, 4}, Hirotsugu Yamamoto^{2, 5}, Takeshi Yasui^{2, 6} ¹Tokushima Univ., Japan, ²JST, ERATO MINOSHIMA Intelligent Optical Synthesizer (IOS), Japan, ³Osaka Univ., Japan, ⁴Tokushima Univ., Japan, ⁵Utsunomiya Univ., Japan, ⁶Tokushima Univ., Japan	Scan-less confocal laser microscope is achieved by a combination of confocal slit with wavelength/space conversion and is applied it to several imaging to demonstrate its high potential.
		BISCp6-24	Multi-focal imaging system by using a programmable spatial light modulator	Chen Yen Lin, Yuan Luo National Taiwan Univ, Taiwan	We have demonstrated that the multiplexed gratings pattern displayed on the digital micro-mirror device (DMD) orLC-SLM at the Fourier plane can separate the diffraction light coming from different depths into different angular directions being recorded by different portions of the single image plane.
		BISCp6-25	Underwater image enhancement algorithm based on granular computing	Yingjuan Xie, Xinnan Fan, Haiyan Xu, Zhuo Zhang, Junfeng Chen Hohai University, China	We proposed an image enhancement algorithm based on granular computing to enhance underwater optical image in this paper. The simulation and experiment results verify the effectiveness of the algorithm.
		BISCp6-26	An edge detection method based artificial bee colony for underwater dam crack image	Zhang zhuo, Fan xinnan, Xie yingjuan, Xu haiyan Hohai University, China	In the proposed model, lateral inhibitory network is firstly presented to enhance edge contrast in complex underwater environment.Then, artificial bee colony is improved to optimize target edge.Experimental results show it is efficient and effective.
		BISCp6-27	Practical image quality evaluation for whole slide imaging scanner	Md Shakhawat Hossain, Toyama Nakamura, Masahiro Yamaguchi Tokyo Institute of Technology, Japan	We propose a method for evaluation of image quality for whole slide imaging system by eliminating false detection due to tissue artefacts. The method enables more efficient and reliable detection of slides to rescan or to score scanned image.
		BISCp6-28	Speckle reconstruction based on oversampling smoothness algorithm	Hui Chen, Yesheng Gao, Xingzhao Liu Shanghai Jiao Tong University, China	Target object image would deteriorate into unrecognizable speckle pattern when encountering with scattering media. In this paper, a method combining correlation method and oversampling smoothness is proposed. It is used for target object reconstruction from scattered speckle pattern.
		BISCp6-29	Speckle reconstruction method based on machine learning	Hui Chen, Yesheng Gao, Xingzhao Liu Shanghai Jiao Tong University, China	Scattering media would deteriorate an object image into unrecognizable speckle pattern. Support vector classification and support vector regression is utilized to reconstruct the object image from speckle pattern.
		BISCp6-30	Fractality of biospeckle pattern observed in blood coagulation process	Naomichi Yokoi¹, Yoshihisa Aizu², Jun Uozumi³ ¹National Institute of Technology, Asahikawa College, Japan, ²Muroran Institute of Technology, Japan, ³Hokkai-Gakuen University, Japan	It has been known that speckle images observed for living bodies illuminated by laser light sometimes show fractal appearances. In this study, we investigate fractality of biospeckle pattern observed in coagulation process of horse blood.
		BISCp6-31	Computational ghost imaging by using complementary illumination patterns	Jung-Ping Liu Feng Chia University, Taiwan	We proposed to use complementary illumination patterns to perform CGI. In addition, we applied Gerchberg-Saxton-like algorithm to optimize the reconstructed image. By this way, the signal-to-noise ratio (SNR) can be significantly reduced.
		BISCp6-32	Preliminary study on X-ray phase-contrast imaging with tilted-grid	Myung-Joon Kwack, Sooyeul Lee, Seung-hoon Chae ETRI, Korea	We present preliminary experimental results of X-ray phase-contrast imaging with tilted-grid. Two-dimensional phase gradient information of a PMMA phantom is successfully defined by employing the tilted-grid in a conventional X-ray imaging setup.
		BISCp6-34	Implementation of a Raspberry-Pi-based LED array microscope for multi-contrast images	Hidenobu Arimoto¹, Wataru Watanabe² ¹AIST, Japan, ²Ritsumeikan University, Japan	The light emitting diode (LED) array microscope enables various multi-contrast imaging such as bright-field, dark-field and differential phase-contrast by various illumination patterns without any expensive optical components.
		BISCp6-35	Image acquisition with smartphone-based LED array microscope	Kazuko Koda¹, Shu Uenoyama¹, Ryo Sugimoto¹, Ryoji Maruyama¹, Hidenobu Arimoto², Wataru Watanabe¹ ¹Ritsumeikan University, Japan, ²AIST, Japan	The light emitting diode (LED) array microscope enables various multi-contrast imaging such as bright-field, dark-field and differential phase-contrast (DPC) by various illumination patterns without any expensive optical components.
		BISCp6-36	Scan-less, line-filed, confocal phase imaging with dual-comb microscopy	Eiji Hase^{1, 2}, Takeo Minamikawa^{1, 2}, Yasuhiro Mizutani^{2, 3}, Tetsuo Iwata^{2, 4}, Hirotsugu Yamamoto^{2, 5}, Takeshi Yasui^{2, 4} ¹Tokushima Univ., Japan, ²JST, ERATO MINOSHIMA Intelligent Optical Synthesizer (IOS), Japan, ³Osaka Univ., Japan, ⁴Tokushima Univ., Japan, ⁵Utsunomiya Univ., Japan	We constructed the scan-less, line-field, confocal microscopy with the phase-contrast modality by a combination of wavelength-to-space-conversion optical frequency comb with dual-comb spectroscopy, and then demonstrate the proof-of-principle experiment of the scan-less confocal phase line-imaging.
		BISCp6-37	Visual search efficiency depending on spatial layout of stimuli in volumetric image	T. Pladere, V. Konosonoka, K. Panke, G. Krumina University of Latvia, Latvia	The spatial layout of stimuli in horizontal and vertical dimension had a bigger impact on visual search performance comparing to the third dimension on a volumetric multi-planar display, also reflected in the subjective difficulty evaluation.
		BISCp6-38	Real-time detection of 192Ir gamma-ray source positon using organic scintillator array sensor in HDR brachytherapy	Young Beom Song, Bongsoo Lee, Sang Hun Shin Chung-Ang University, Korea, Republic of	In this study, we fabricated an organic scintillator array sensor (OSAS) based the array of organic scintillators. The scintillator array of OSAS for detecting positions of 192Ir gamma-ray source was fabricated using four types of organic scintillators, which emit the scintillating lights of different wavelength, respectively.
		BISCp6-39	Electron beam addressable potentiometric sensor for ion distribution imaging with high resolution	Wataru Inami, Yoshimasa Kawata Shizuoka Univ, Japan	We have developed an electron beam addressable potentiometric sensor to improve the spatial resolution. Ion sensors are widely used in the fields of medical and life science, food and material development, environmental protection and so on. However, the spatial resolution of the ion distribution imaging sensor is limited by the diffraction limit of light or microfabrication technology.
		BISCp6-40	Design of add-on optics for optimization of cot endoscope camera for epiduroscopic surgery	Khanh Phuong Tran Intelligent Systems Research Institute, Korea, Republic of	This paper presents a method for designing an add-on lens assembly to optimize the performance of Chip-on-the-Tip (COT) endoscope. In particular, an add-on lens assembly is designed here attributes to a commercially available COT camera, NanEye, in such that provides a FOV of 110º and DOF of 1.5 mm to 8 mm under the refractive index of water.
		BISCp6-41	Disturbance location algorithm of the single-core fiber optic sensor based on frequency domain	Haiyan Xu, Yingjuan Xie Hohai Univ, China	A novel distributed fiber-optic sensor based on Wavelength Division Multiplex (WDM) for determining the position of disturbances is presented. Theory analysis and experiment results show that the proposed algorithm can realize the detection and location of the multipoint disturb signals rapidly and effectively.
		BISCp6-42	Design optimization of a single-mode microring resonator for label-free detection of biomarkers within a tunable spectral range of 2 nm	Prabodh Panindre¹, N. S. Susan Mousavi², Sunil Kumar^{1, 3} ¹New York University, USA, ²Institute for Research in Fundamental Sciences, Iran, ³New York University Abu Dhabi, United Arab Emirates	The electromagnetic frequency domain parametric analysis using finite element numerical technique quantifies the effect of geometrical design parameters of microring resonator on its optical characteristics to optimize its performance for label-free detection of nano-biomarkers.
		BISCp6-43	Low dose of narrow-band ultraviolet B lamp for improving vitamin D synthesis with minimum skin damage	Lin Yu-Hsuan¹, Yi-Wen Chiu^{2, 3}, Ming-Yen Lin^{2, 3}, Siao-Ping Tsai², Feng-Xuan Jian³, Shang-Jyh Hwang^{2, 3}, Kuo-Cheng Huang¹ ¹National Applied Research Laboratories, Taiwan, ²Kaohsiung Medical University Hospital, Taiwan, ³Kaohsiung Medical University, Taiwan	This study proposes that the UV irradiation with a wavelength of 310-320 nm can effectively improve the vitamin D synthesis and minimize the skin damage.
		BISCp6-44	Photostability study of CdTe quantum dots using laser induced fluorescence	Souad A. Elfeky, A.El-Hussein The National Institute of Laser Enhanced Science, Cairo University, Egypt	Semiconductor quantum dots (QDs) having high quantum yields and unique photostability. This research studies the optical properties of the synthesized CdTe QDs with two different sizes using Laser-induced fluorescence (LIF) for investigating their photostability.
		BISCp6-45	Edge contrast enhancement at multiple planes using Forked Shaped defocus grating	Sunil Vyas, Chen Yen Lin, Yuan Luo National Taiwan Univ, Taiwan	We report on the implementation of spiral phase contrast imaging at multiple planes using forked-shaped defocus grating. The dual function of grating helps in simultaneous generation of multiple edge enhanced images corresponding to different depths.
		BISCp6-46	Orthogonal functional system for finite Fresnel transform	Tomohiro Aoyagi, Kouichi Ohtsubo, Nobuo Aoyagi Toyo University, Japan	The Fresnel transform has been studied mathematically and revealed the topological properties in Hilbert space. Main aim is to reveal the property of band-limited function. We seek the function that its total power is maximized in finite Fresnel transform plane, on condition that an input signal is zero outside the bounded region.
		BISCp6-47	Nanoscale three-dimensional imaging of biological tissue with X-ray holographic tomography	Alexandra Pacureanu, Julio Caesar da Silva, Yang Yang, Sylvain Bohic, Peter Cloetens European Synchrotron Radiation Facility, France	Enabling exploration of biological tissue in three-dimensions at sub-cellular scale is instrumental for advancing our understanding of biological systems and for finding better ways to cope with diseases. Over the last few years, remarkable advances in microscopy facilitated probing cells and tissues at the nanometer scale but many limitations are yet to be overcome. Here we present a novel technique which enables label-free volume imaging of biological tissue with pixel sizes down to 25 nm while maintaining extensive sample coverage. X-ray holographic nanotomography is a full-field 3D imaging technique which benefits from the deep penetration of X-rays and the powerful mechanism of phase contrast. By using cryogenic sample preservation, the tissue can be investigated close to the native state. The unprecedented data created by this technique opens new avenues in life sciences research.

BISC7: Optical Diagnosis and Treatment
Chair: Joseph Rosen(Ben-Gurion Univ of the Negev, Israel)

Room 419

Start	End	Session No.	Title	Speaker	Description
15:00	15:15	BISC7-1	Quantitative evaluation of healing degree in injured tendons based on orientation analysis of collagen fibers by using Fourier-transform second-harmonic-generation microscopy and its relationship to mechanical property	Eiji Hase¹, Takeo Minamikawa², Katsuya Sato², Daisuke Yonekura², Mitsuhiko Takahashi³, Takeshi Yasui² ¹Japan Sychrotron Radiation Reasearch Institute, Japan, ²Tokushima Univ., Japan, ³Tokushima Pref. Cent. Hosp., Japan	We used Fourier-transform second-harmonic-generation (FT-SHG) microscopy to analyze the orientation of collagen fibers in healing rabbit tendons recovered from an artificial transection and assessed the correlation between the orientation parameter and Young’s modulus.
15:15	15:30	BISC7-2	Noninvasive estimation of light scattering and hemoglobin concentration in mice cutaneous carcinogenesis through multispectral imaging	Izumi Nishidate¹, Satoko Kawauchi², Shunichi Sato² ¹Tokyo Univ of Agriculture and Technology, Japan, ²National Defense Medical College Research Institute, Japan	The proposed multispectral diffuse reflectance images acquired at isosbestic wavelengths of hemoglobin were able to estimate the total hemoglobin concentration and tissue scattering parameter of mice skin during cutaneous two-stage chemical carcinogenesis.
15:30	15:45	BISC7-3	Characterization of cancer metastasis in model mice by multiphoton microscopy and Raman Spectroscopy	Yusuke Oshima, Shigehiro Koga, Yuji Watanabe Ehime Univ, Japan	In this study, we investigate molecular dynamics in both cancer cells and their environment in xenograft models and spontaneous metastasis models using Raman spectroscopy and nonlinear optical imaging. We are also constructing a custom-designed Raman spectral imaging system to reveal the metastasis process and to evaluate therapeutics toward the clinical application of the technique.
15:45	16:00	BISC7-4	Glucose sensing in the presence of scattering particles using decomposition of partial Mueller matrix	Pradipta Mukherjee, Yukitoshi Otani Utsunomiya Univ, Japan	A Partial Mueller matrix polarimeter retrieves a subset of sample polarization properties that can be useful for specific measurement. A partial Mueller matrix decomposition method is proposed to retrieve the optical rotation and depolarization simultaneously for measuring glucose concentration in the presence of scattering. A dual-photoelastic-modulator based Mueller matrix polarimeter is designed for this purpose. We verify the proposed decomposition method by measuring different glucose concentrations mixed with scattering particles.
16:00	16:15	BISC7-5	Quantitative in situ time-series evaluation of osteoblastic collagen synthesis under cyclic strain using second-harmonic-generation microscopy	Katsuya Sato¹, Eiji Hase², Takeo Minamikawa¹, Takeshi Yasui¹ ¹Tokushima University, Japan, ²Japan synchrotron radiation research institute, Japan	The aim of this study is to provide an in situ method to non-invasively monitor osteoblastic collagen synthesis under mechanical stimulation. We applied second-harmonic-generation (SHG) microscopy to monitor the collagen fibers produced by osteoblast-like cells. To evaluate the influence of mechanical stimulation on collagen synthesis and maturation, we compared SHG images of osteoblast-produced collagen fibers with and without a cyclic stretch stimulus. We acquired SHG images every 7 days for 3 weeks at different stimulus conditions. Image analysis of the average SHG intensity indicated that the amount of osteoblastic collagen synthesis was significantly enhanced by the cyclic stretch compared with the the non-stretched condition, while there was no significant difference between the two stimulus conditions. Furthermore, the maturity of the collagen fibers was not affected in the early stage of bone formation by the mechanical stimulus.
16:15	16:30	BISC7-6	Fluid dynamic modeling and comparison of the intraocular pressure changes in eyes with SMILE and LASIK	Kuo-Jen Wang¹, Cheliang Tsai¹, I-Jong Wang² ¹Crystalvue Medical Corp, Taiwan, ²National Taiwan University Hospital, Taiwan	We developed an intraocular pressure analytic model utilizing fluid dynamics, solid mechanics, and ray-tracing technique to simulate the air-puff noncontact tonometry for post-SMILE and post-LASIK IOP measurement.
16:30	16:45	BISC7-7	Enhanced therapeutic effect of an antitumor agent on malignant glioma in rats by photomechanical wave-based transvascular drug delivery	Yumiko Koshi¹, Izumi Nishidate¹, Shunichi Sato² ¹Tokyo University of Agriculture and Technology, Japan, ²National Defense Medical College Research Institute, Japan	We used a photomechanical wave (PMW) to enhance the delivery efficiency of an antitumor drug, temozolomide (TMZ) in a F98 rat glioma model, and showed that therapeutic effect of TMZ was improved by PMW application.
16:45	17:00		Closing Remark & Award Ceremony	Osamu Matoba Kobe University, Japan